Biotechnology Ion Exchange Chromatography Improvement

Abstract

This research investigates the influence of different stop collection percentages on ion exchange chromatography in laboratory-scale separations. A direct association was seen between reduced stop collection (percentages of peak max) and increased chromatographic area under the curves, resulting in enhanced substance elution. Four (4) experimental runs showed linear behavior (97%, 95%, 93%, 90%, and 88% peak max). The presence of variability among runs serves to emphasize the susceptibility of outcomes, hence it is important to consider the control of key parameters such as chromatography process conditions and sample stability. The suggestions for future research include (1) adjusting column conditions and (2) investigating causes of variability to enhance reproducibility. This study offers significant contributions to the productivity of a blockbuster drug, by enhancing the uses of ion exchange chromatography. The supporting data was generated using a scaled-down model of the large-scale process operations. Key Terms ⎯Chromatography, Ion Exchange, Scale-Down Model, Stop Collection.